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In order to satisfy the requirements of thermal comfort of occupants with different clothes, taking a room heated by stratum ventilation as the research object, and optimizing the ventilation parameters (i.e., supply air velocity, supply vane angle, and supply air temperature) via an improved TOPSIS method under considering different clothing insulations. To reduce costs of computational, the energy utilization coefficient, PMV/PPD, mean age of air, and vertical air temperature difference of head and ankle were taken as ventilation performance evaluation criteria, and supply vane angle, supply air velocity, supply air temperature, and clothing insulation were design parameters. The CFD simulation analysis is performed by means of the central composite design response surface method to obtain the functional relationship between the ventilation performances and the design parameters and was then used to evaluate the ventilation performance of all possible operations. The results demonstrate that optimization on the ventilation parameters via the improved TOPSIS method can significantly improve ventilation performance. The vertical air temperature difference between head and ankle levels, the mean age of air, and the PPD decreased by 48.17%, 22.46%, and 37.08% on average, respectively, and an average increase in the energy utilization coefficient by 20.42%.