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In order to effectively monitor the health of broilers in the farm, it is necessary to collect live audio signals to identify vital signs information such as chirping and coughing. So, this research designed an audio collection system for broiler farm based on wireless transmission technology. The system consists of the collection terminal, the wireless communication module and the remote server. The wireless communication module is used as the communication medium between the collection terminal and the remote server to establish a Socket connection between the two. When the remote transmission is normal, the collection terminal works in a timing or real-time working mode, and the collected audio signal is filtered by Bandpass and Spectral Subtraction and sent to the remote server for storage. When the remote transmission is abnormal, the collection terminal saves the audio signals collected and processed in different working modes to the SD card storage module. The test results show that the collection terminal can collect 5 min-long audio signals according to the set working mode. The collected audio signals can be quickly transmitted to the remote server for storage within 10 s. The average increase in signal-to-noise ratio of the audio signals before and after filtering is 1.862. Counting the audio signals transmitted and saved within a month, it is concluded that the total packet loss rate is within 0.3%. The system provides an important source of audio (data) for the follow-up study of broiler health monitoring, so it has high practical value.