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The development of bus technology brings new requirements of distributed, information and network to the cable tester, and the increase of the number of test lines in the working process of the distributed cable tester also puts forward higher requirements on the stability and communication efficiency of the bus data communication. According to the application requirements of distributed system in cable testing, the TTCAN application layer protocol and its system matrix of distributed cable testing instrument are designed and optimized. The system matrix formed by periodic messages in distributed system communication is optimized by genetic algorithm and improved differential evolution algorithm successively, and the dynamic priority algorithm based on relaxation degree is adopted for the non-periodic messages. Experiments in MATLAB simulation environment show that the improved difference algorithm can calculate the optimization matrix more quickly and stably than the genetic algorithm, and the data transmission efficiency of TTCAN bus after scheduling optimization is significantly improved. In this paper, the communication efficiency and stability of the system bus are improved effectively by the intelligent optimization algorithm.