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As a vital sign monitoring equipment, physical fitness monitor is of great help to assist college students to complete physical training. Based on STM32 single chip microcomputer technology, a college students" physical fitness monitoring equipment integrating a variety of sensors is designed and developed. It can effectively detect the information including heart rate detection, human steps, lap speed, calorie consumption and so on. The design uses STM32 as the main control chip and adopts the modular design idea. In the aspect of system hardware design, it includes STM32 control system, heart rate acquisition circuit, three-axis sensor circuit, display circuit and key circuit. At the same time, the software module is divided into initialization subroutine, heart rate acquisition subroutine, step acquisition subroutine, display subroutine and so on. Proteus simulation results show that the physical fitness monitoring system designed with STM32 single chip microcomputer technology can run stably and meet the needs of physical fitness monitoring tasks. The experimental results meet the expectations. STM32 single chip microcomputer provides technical reference for sports research informatization.