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Aiming at the problems of label capacity limitation and communication conflict in UWB location, an improved location method based on flood mechanism quasi-synchronization was proposed. By setting the master-slave base station, the clock synchronization between the base station and the tag is realized by using the flooding mechanism to transfer the quasi-synchronous message step by step, which meets the requirement of the TOA algorithm in the DS-TWR method for the clock error in the communication, thus improving the ranging accuracy. At the same time, the unique time slot is allocated to the tag base station pair through the multi-hash operation, which effectively improves the communication success rate under the condition of multi-tags. The experimental results show that the average communication success rate of the improved system is increased by 16%, the energy consumption of the tag is reduced by 30%, and the amount of data acquired per unit time is increased by 27.8% under the condition of multi-tag. The system can improve the communication efficiency and reduce the energy consumption, which has a high engineering application value.