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In order to improve the accuracy of transcranial electrical stimulation therapy and realize personalized therapy, an eight channels electrophysiology regulation system by combining transcranial stimulation with EEG was designed which can monitor and analyze the EEG signals before and after transcranial electrical stimulation, and adjust the parameters of transcranial electrical stimulation according to the EEG signals and evaluate the effect of transcranial electrical stimulation therapy. Using STM32F407VET6 as the core control device to achieve transcranial electrical stimulation and EEG signal acquisition function switch. Software uses C# and MATLAB mixed programming. The upper computer can display the EEG waveform, analyse EEG spectrum and adjust the parameter of electrical stimulus. The experimental results show that the EEG signals are successfully collected and digitally processed before and after the output of transcranial electrical stimulation. The blinking waveform and resting state α waveform are obvious. The system can output DC electric stimulation, standard square wave, standard triangular wave and user-defined waveforms of alternating current stimulation. The range of stimulus current is 0-2 mA and the frequency of the stimulus is in the range 0-1000 Hz.