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Pulse measurement is one of the basic monitoring work in medical care. The traditional pulse sensor mostly uses finger pulse, ear pulse and other methods, but it also has the disadvantages of difficult to effectively maintain and inaccurate data. In order to effectively solve the above problems, a digital pulse measuring instrument based on STC89C52 single chip microcomputer is designed. The infrared tube sensor and photoelectric conversion are used to sense the weak fluctuation of fingers and realize signal reception. This design uses STC89C52 microcontroller as the core of the controller, and outputs square wave through the heart rate sensor. When the microcontroller receives the corresponding pulse wave, the digital tube can count and send signals to the receiving end to achieve the counting and monitoring functions of the pulse meter. The simulation results show that through the relevant debugging and use, the pulse measuring instrument has successfully realized the corresponding functions in the design, and has potential application value.