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In order to realize the precise control of the multi-functional chromatographic separation device and accelerate the purification and separation speed of simulated moving bed chromatography, a multi-functional chromatographic separation device control system based on PROFIBUS DP fieldbus was designed. Taking STM32F103 microprocessor as the bottom control equipment, connecting PROFIBUS-DP protocol chip, vpc3 and RS-485 interface circuit, converting slave station address circuit and setting application module, using analog mobile bed control circuit, the hardware circuit design of PROFIBUS DP field bus is completed. The gradient SMB chromatography was established. The asynchronous switching mode was adopted to control the actual operation behavior of variable concentration feed. The separation and processing simulated the moving bed multi-functional chromatography. Combined with the control principle of the multi-functional chromatographic separation device, the matrix effect form in the chromatographic separation process was analyzed by using gas chromatography, and the control system software design of the multifunctional chromatographic separation device was realized. The experimental results show that the simulated moving bed chromatographic purification and separation speed based on PROFIBUS DP fieldbus control system is fast, which can effectively meet the operational requirements of precision control of multi-functional chromatographic separation device.