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At present, heart disease is the number one killer causing deaths in our country. However, traditional ECG signal acquisition systems and terminals have problems such as missing detection data, high costs, and narrowly applicable populations. Based on the principles of modular, low energy consumption, and high performance, this paper designs a real-time ECG signal acquisition system based on ARM and WIFI technology. This article gives the introduction of each functional module of the ECG signal real-time detection system and the function realization process. The upper computer uses TCP Client as the client, MCU as the lower computer, and WIFI technology as the communication medium. The main control module and heart are designed. The electrical heart rate acquisition module, LCD display module, and communication module realize the real-time acquisition and transmission of ECG signals. The system test results verify the practicability, rationality, and effectiveness of the ECG signal real-time detection system. The experimental results reach the expected goal, which can effectively improve the accuracy of the detection results and provide a real-time and reliable detection for the detection and prevention of heart diseases. platform.