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In order to ensure the safety of water quality and realize the online monitoring of organic pollutants in water, based on the principle of fluorescence detection, an online fluorescence monitor for organic matter in water was designed. The monitor includes fluorescence detection device, flow path and sampling module, power supply module, industrial computer and touch screen, database and analysis software, and data transmission module. The structure of fluorescence detection device is introduced in detail. The optical, electrical and structural parameters of the monitor are given, and its performance is evaluated. The spectrum detection range of the monitor is 300nm-750nm, and the detection time is less than 10s. Tryptophan aqueous solution was used as the sample for the experimental test. The linearity determination coefficient R2 of the monitor is 0.95, and the relative standard deviation (RSD) of fluorescence intensity test results is 2.68%. The remote data transmission of the monitor is reliable and stable, and the maintenance cycle is once a month. The monitor can monitor the organic pollutants in water in real time, and can obtain the water quality information in time. It has the advantages of high detection speed and no detection reagent. And it can effectively avoid the secondary pollution caused by chemical reagents.