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Based on nuclear pipeline evaluation system, the fault diagnosis and control design of the core hardware system is studied. The hardware conditioning module is designed by means of program-controlled amplification, phantom power supply, fault diagnosis and other circuits. The hardware system control and fault diagnosis function are realized by Keil software programming STM32 chip embedded software, and use the designed serial communication protocol communicating with the software system through RS232. The hardware automatic test platform and fault diagnosis monitoring program are developed through Labview software to ensure system reliability, improve test efficiency and reduce labor cost. The designed hardware control and fault diagnosis module meets the requirements and has been successfully applied in the pipeline evaluation system of a certain nuclear power plant exported to other countries, providing guarantee for the safe, economical and reliable operation of the nuclear power plant.