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To overcome the shortage of commercial acoustic emission detection systems, a multi-channel acoustic emission detection system was designed and developed based on virtual instrument technology. A universal high-speed synchronous data acquisition card was adopted for sampling four channels of acoustic emission signals. The data acquisition frequency of each channel can be configured as high as 125MHz and the sampling length can be adjusted flexibly. LabVIEW was used as the platform for developing the software to realize the control of data acquisition process, preprocessing of acquisition data, waveform display and data storage functions. Both one-dimensional and two-dimensional lead breaking experiments were carried out with the developed detection system. The lead breaking positions were located by processing the experimental data and the sources of positioning error were analyzed from multiple perspectives. Finally, the performance of the system was evaluated qualitatively and quantitatively according to industry standards. The experimental results have demonstrated that the location error of AE source is less than 5% of the maximum sensor spacing in the sensor array, verified the availability of the developed system, and shown that the hardware conditions, software functions and positioning accuracy meet the industry standards. The design scheme, experimental method and data processing method of the acoustic emission detection system will provide useful reference for the research and development of industrial field acoustic emission detection systems.