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Chillers usually account for the main part of the total energy consumption of building systems, the control of the number of chiller operations plays an important role in achieving energy savings in air conditioning systems. Aiming at the problem of unreasonable number of chillers and load distribution of the combined operation of multiple chillers, the article used cluster analysis of load values obtained from TRNSYS simulation to determine the number of chillers corresponding to different load values, and propose a chiller sequencing optimization control method to improve the sequencing control method to achieve the reasonable operation and energy saving of chillers. Take a large office building as an example, the total energy consumption of the chillers operation before and after the optimization control is compared. The experimental results show that compared with the sequential control, the total energy consumption of the chillers after the optimal control of the two working days is 126.1 kW and 342.5 kW, respectively, and the energy saving rates are 4.15% and 5.22%, respectively. By using the chiller sequencing optimization control, can meet the cold load demand of the building and reduce the energy consumption of the system, which has practical application value in energy saving of the air conditioning system.