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Concern over the software and hardware environment of domestic LOONGSON platform with NeoKylin operating system, the basic framework, key data structure and loading mode of PXI device driver under Linux are analyzed . Typical PXI device driver modules, such as PXI device initialization, data read-write, device control, interrupt handling are also analyzed in detail. The design process of PXI device driver under Linux is discussed. And the driver interface for application layer which is based on the Instrument Software Architecture and simplifies the design of application layer software is provided. The design provides a good application case for the development of PXI equipment software based on LOONGSON platform and NeoKylin operating system. The actual verification results show that in the field of measurement and control, the combination of LOONGSON Platform and NeoKylin successfully combines open source software and PXI hardware. Beijing AMC corporation takes the lead in establishing a self- controllable PXI universal test platform in China.