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The design of a resistance measurement system for satellite thermal control circuit based on virtual instrument has been presented in this paper. The digital multi-meter and a matrix switch module are integrated in NI PXI platform, and the software is programmed in Labview development environment. The insulation and resistance measurement of satellite thermal control circuit which contains 120 access points can be implemented quickly and automatically through software controlled switch alternation. Data management and report generation function are also included. The measured results have been proved to be accurate and reliable through comparing with the calibrated resistance, the relative error is less than 1%. The system is highly integrated and flexible to use. It can improve the measurement efficiency, save about 80% test time, and has high application value.