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Source seeking become a hot issue at home and abroad, in order to study the phototaxis of mobile robots in the laboratory, research on source seeking theory methods, design a mobile robot platform, verify the feasibility of the phototaxis theory by this platform. Firstly, using diagonal control strategy and gradient adaptive extremum search algorithm, the phototaxis experiments are carried out, respectively, to complete the light source seeking of mobile robot. Secondly, MeanShift target tracking algorithm is used to track the moving trajectory of mobile robot. Finally, draw the contours of light source intensity distribution and analyze the motion trajectory of the mobile robot. Compared with the diagonal control strategy, the gradient adaptive extremum seeking algorithm adjusts the feedback gain parameters according to the current regional gradient value, which improve the efficiency of source seeking, and verify the effectiveness and stability of the algorithm on the platform.