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In view of the current highway traffic accident, it is difficult to accurately and quickly locate the accident, which leads to the lag of the rescue response. A vehicle collision monitoring and positioning system based on the Internet of things is proposed, the collision physical signal can be perceived by intelligent bolt acceleration sensor. LoRa technology and the 4G transmission were combined to realize the remote synchronous real-time transmission of the signal in the data transmission module. Based on the threshold isolation algorithm of detection system platform, the signal of interference and collision can be distinguished effectively. Experimental results show that the detection system has realized the effective scope of monitoring sections with real-time, which the information of traffic accident about specific location, severity and time of occurrence can be judged effectively within 20 seconds. Which has important directive significance to improve the efficiency of rescue response and ensure the safety of life.