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In order to meet the needs of multi-channel UART parallel communication in engineering application, this paper designed a multi-channel UART parallel transmission system based on STM32F429. The system makes full use of the eight UARTs and network interfaces inside the processor, DMA is used to receive UART data in a circular buffer, and this solves the problem of data frame loss and processor occupation time caused by querying or interrupting mode in concurrent reception. For different protocol frames, the method of timeout judgement is adopted to avoid subsequent reception problems once the UART receives incorrect data length. The lwIP protocol stack is used for network transmission. The design realizes the simultaneous acquisition seven channels card number and uploads data to the host computer through an UART or a network port, the test shows that there is no data frame loss during the transmission of the system. The system has no external UART circuit, which reduces the volume of the whole circuit and improves the reliability and stability of the system.