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In order to solve the problem of the material board automatic assembly during manufacturing process, an inspection system of robot positioning assembly based on machine vision is proposed and experience platform is made. The system grabs the images of the tray by using the industry camera, transports the image information to industry computer, uses the image processing program to reduce the noise by pretreatment, extracts the image features by using blob analysis, recognizes the hole position of the material board, and controls the robot to move to the correct position achieving automatic position assembly. Experience result shows the system has a high accuracy and meets the need of the industry.