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Aiming at the problem of path optimization of mobile robot in a wide range of environments, this paper proposed a route and time optimization strategy based on RFID nodes’ navigation instruction. In a topological map environment, using the RFID information nodes to label critical locations such as corridors, intersections, corners and so on in the environment and guide the robot with navigation instruction. The robots are divided into three patrols to solve the total distance of the shortest and balanced route. Set the robot at the node's dwell time, study the path optimization method for traversal all nodes at a given time. This study provides a path optimization scheme for large-scale placement of RFID nodes to guide robots.