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In the condition of breeding livestock in open circumstance, the livestock were easily lost, difficult to locate and statistic. In order to solve the problems, a new kind rear livestock location system was designed using RFID and UAV.The whole system consists of three function components:data acquisition, data processing, terminal display. By binding an active RFID tag to each livestock, with UAV which equipped a RFID reader and a mobile phone to perform automatic cruise, the RFID data together with the latitude and longitude data could be send to the data server after pretreated by the phone. In the server, the data got a further analysis and processing. Then, the livestock position data and statistical data would be display in the breeder's mobile phone, facilitated the breeder to carry out livestock management, to avoid loss. At last, with the experimental data and the results analysis, the system's location result was within the estimation error range, meet the performance of high accuracy and feasibility. Compared with the traditional solution such as constructing fence, the system has the advantages of low cost, convenient, high feasibility.