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Linear guide rail is an important component of the numerical control technology which is very important to the degree of accuracy. With the core of MSP430 single chip microcomputer, a precise positioning device for linear guide rail is designed. The device adopts two phase four wire ST57 type step motor and 60CM screw thread rod to form a linear guide rail group which use M7128 driver to drive the stepper motor, using the program of PWM positioning algorithm to precisely control motor speed.MSP430 IO port control stepper motor positive inversion,making the stepper motor driven screw thread rod rotation, so that the object on the rolling screw to move back and forth, and the computer shows the value of the current displacement. Experiments show that the positioning accuracy is 0.01 cm, which has high reliability and accuracy, low cost and good stability.