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Total pressure is an important parameter in the wind tunnel control, and its regulation performance is an important index to meet the requirements of the control system. In order to improve the total pressure control level of transonic and supersonic wind tunnel, the total pressure control strategy needs to be designed. Aiming at the quickness and accuracy requirements of total pressure control system that been proposed by a certain transonic and supersonic wind tunnel, we used the subsection control mode, cascade control and intelligent PID control in the control system, and proceeding the model identification of the total pressure system of flow field adjustment section by the MATLAB system identification toolbox. We putted forward a new method that using the genetic algorithm in the PID controller parameter setting of the wind tunnel flow field adjustment section. The paper focuses on introducing the principle of PID control based on genetic algorithm and the parameter setting step, in addition, improving the genetic operators of genetic algorithm. System simulation and the wind tunnel operation show that this method has the better control performance than the conventional PID parameter setting and optimization method, which satisfies the requirement of control system and provides a new way in the future .