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In view of the Fuzzy C-Means clustering algorithm’s single membership degree can’t fully describe the images uncertainty, and ignore the pixel spatial relations in the process of clustering, here put forward a kind of image segmentation algorithm based on spatial information and intuitionistic fuzzy sets. The algorithm select the template of 3×3 computing neighborhood pixels within the grayscale average; and introduce the weight to control the gray information and spatial information, at the same time using hesitation degree to update the membership function of intuitionistic fuzzy sets. In view of the common standard image simulation experiment results show that the algorithm can keep the details of the image information better and obtain a more ideal image segmentation results.