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To solve the problem of equipment’ independence, A completely domestic multi-channel analog signal acquisition circuit based on domestic CPU and ADC is designed in this essay. In order to improve the low precision of domestic analog circuit, recursive average filter method and least square estimation method were used to reduce the random error, the inherent offset error and gain error.It could be concluded from the experiment results that the acquisition circuit could acquire multi-channel analog signal simultaneously, and had high acquisition precision. The biggest sample error was reduced from 0.8% to 0.12%. The circuit was of high reliability and of 100% domestic rate, making it have good prospect for application.