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Due to the flexibility of PXI Devices, users need to participate in the configuration process. In order to offer convenience to the users, improve the security and veracity, a new method of PXI device access based on VISA has been brought up. By fixing up the serial ID to the configuration register of PXI devices, then get the device resource descriptor automatically by the serial ID and access the devices. The application result shows, the method can access to the PXI devices, and avoid the configuration process of users, realize the automatical configuration of PXI resources, improve the security and veracity, has great value of actual application.