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Radio frequency identification (RFID) technology has been developing rapidly from the rising of the Internet of things, UHF RFID technology has been widely used because of its long recognition distance, fast recognition speed, strong anti collision capability. Compared with the RFID technology, the RFID testing technology is relatively laggard, and it mainly carries on the qualitative test based on reference tag or reference reader. In order to realize the quantitative measurement of the RF parameters and protocol conformance of the RFID reader, an UHF RFID reader tester based on RF processing and digital software-defined radio (SDR) is designed. The tester is a portable device, and it has many characteristics such as small volume, low power consumption, light weight, strong function, simple operation, and it can meet the UHF RFID reader’s test requirements of mass production and field support. 