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Bio_electrical impedance is an important electrical parameter of biology tissue,bio_electrical impedance measurement and analysis has important research and application value on the bio_medical engineering。Using four electrode method,designing a multi-frequency excitation bio_electrical impedance measurement system,making use of AFE4300 produce 16-128 kHz adjustable excitation signal source,putting the current electrodes on the test of biological tissue, through the IQ demodulation, can calculate the measured impedance modulus value and angle .When the excitation signal is 128 kHz, the system has the maximum error,the error is 2.07%.And with the increase of excitation frequency, modulus and phase Angle measurement error reverse the trend so that in different applications, choose different excitation frequency can improve the measurement precision of the system.