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Based on FPGA and MCU technology, a multi-channel signal optical fiber transmission system is designed, which makes use of MCU for high-precision analog data acquisition and bidirectional transmission of a communication signal；FPGA for processing and transmission of multi-channel complex signals. Experiments show that:the system not only can transmit multi-channel analog and digital signals, low-speed digital signal and high-speed pulse signal, but also can realize the bidirectional CAN communication. Compared with the existing optical fiber transmission system, the multi-channel signal optical fiber transmission system realizes data acquisition of multi-channel complex signals, also realizes bi-directional transmission of multiple high-capacity data with a fiber. On the one hand reduces the volume of the product, on the other hand to reduce the product cost.