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To facilitate the CCD camera to capture images under the microscope, control of the microscope is studied .Design a method for realizing automatic control the microscope. Electrical control to adjust the focus use THB6128 chip stepper motor drive, use PT4115 chip control LED lights achieve the lights dim lighting adjustment, based on LabVIEW virtual instrument platform to develop the main control program to build the peripheral hardware, realize the automatic control of microscope. Experiments prove that the PC sends instructions to the lower computer through serial data communication, by adjusting the LED lamp light and dark as well as a stepper motor driven rotation of the stage moving up and down, achieved obtain a clear image at a suitable lighting and focus. Make microscope control more simplified and precise, easy image acquisition.