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In order to meet uncompressed High Definition Television data in real-time collection system,and solve the problem of data loss caused by small capacity and low bandwidth of common data buffer, a high speed and large capacity data buffer based on DDR3 SDRAM is proposed in this paper; The method includes the key techniques such as data transmission in different clock domain, high bandwidth data memory design and priority arbitration on data bus;data buffer can run at a high data rate up to 400Mbytes/s.The experimental proved that the data buffer can be applied to uncompressed High Definition Television collection system .