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High frequency and high voltage generator is one of the core components of the X-ray machine, whose role is to provide DC high voltage for the high-pressure tube so that the tube can output energy-concentrated X-ray, and the DC voltage value is a key parameter of X-ray machines. When used in different individuals and different parts of the body, medical X-ray machines often require different tube voltage rating (kV value) to control the amount of radiation X-rays. Therefore, the output voltage of the tube must be highly precise and adjustable. The technology of full-bridge series-parallel resonant inverse transform combining with the pulse frequency modulation system is used in the design to adjust the output of high voltage power supply, and the output of high-voltage DC power supplied to the tube for ranges from 40kV to 150kV. The control part of the system uses the STM32 microcontrollers to detect and control high-voltage DC power. The experimental results show that the output of the DC high power from the high frequency and high voltage generator designed in this paper has a relative error less than 5%, high accuracy and good stability, and it can satisfy the work requirements of medical X-ray machine which has a good value.