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The anti-aliasing filter in data collection is a very important consideration in circuit design. In the data acquisition system, for an analog signal of certain bandwidth, the sampling rate must satisfy the Nyquist sampling theory, otherwise it will cause aliasing. This will lead to the original signal cannot be restored from the sampled signal, seriously affects the measurement result. The design of the signal conditioning and A/D acquisition part have adopted anti-aliasing design, FPGA using XC3S400 of the XILINX company as the main controller. Integrating all aspects, we propose the anti-aliasing filter design methods for the high-precision data acquisition system, give a brief introduction AD sampling and the sequential design of channel multiplexers’ switching. This provides a very useful reference for the system.