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A kind of data acquisition and preprocessing scheme based on Nios II processor that is used in fiber strapdown inertial navigation system is put forward, with the situation of repetitive design and low efficiency exists in the traditional design of navigation computer in the inertial navigation system. The IP core of data acquisition of fiber optic gyroscope and accelerometer is designed using verilog language, and with the use of SOPC (system on a programmable chip) technology, the Nios II softcore processor is customized, and the FIR low-pass filter of 16 order is designed. Through the static experiments of fiber strapdown inertial navigation system, the results show the accuracy of IP core of data acquisition and the validity of FIR low-pass filter.