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According to medium environment safety and system complexity of weighing method at low temperature flow calibration system, measurement and control system at low temperature calibration was developed based on the industrial control computer and card. System execution was automated.The paper introduces the overall design ideas of calibration system from the principle、control requirements and work flow, the hardware design was realized from the system’s hardware composition and function,the main key technologies of the software was overcomed from consider visualizing and modular software.Through the hardware and software of the system debugging,the system function is complete,reliable,convenient,meet the test requirements of flowmeter under low temperature.