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Owing to the given cold chain logistics monitoring system paying attention to the system software and hardware or the compress and fusion manage, not considering the feasibility, energy-saving and short-part routing protocol design. Therefore, a cold chain logistics monitoring system based on RFID and Low Duty Cycle Opportunistic routing is designed. Firstly, the main system framework figure is given and the hardware of the parts including RFID label, label reader, node and sink node are designed. Then the Opportunistic routing with Low Duty Cycle is introduced, the sink node broadcasting the Hello data packet to get the data transferring direction and neighbor selective node set, and the states for the neighbor selective node set are predicted to obtain the final selective nodes with opportunity. The experiment is tested to show the system in this paper can monitoring the system effectively, and compared with the other methods, it has the short path of transfer, high data packet reaching rate and short delay time, so it is with big priority.