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For detecting the real time temperature gear-box of automotive transmission,enhancing the security in the moving process of automotive,the temperature sampling system was designed based on controller of STM32.The temperature of lubricating oil in gear case was detected by PT100,the temperatrue data was sent to the upper monitor by the serial port of the control card STM32,the temperature curve could displaied by monitoring interface ,which was worked out by LabVIEW,and the collected data was keep in Excle.Do the Kalman filtering to the data by Matlab,so that the magnetic interference signal filtered availably,achieved the differential coefficient of the temperatrue curve in unit time in the meantime,detected whether the oil temperature was normal.The experimental result showed that,the dynamic property of the system of automotive axle temperature is high,the response time t<50ms,the temperature resolution δ≤0.01℃,so that the operating requirement of the axle temperature of automotive transmission system was satisfied.