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SGT320E three-axis turntable platform is widely used in the field of inertial sensors calibration and the performance testing of inertial measurement unit and attitude and heading reference system. In the application, one of the key issue is that how to achieve data synchronously from the three-axis turntable platform and the test device. Based on the synchronous pulse mechanism, three methods of synchronous data output were put forward. By improving the multithreading serial communication class CSerialPort and using Microsoft visual studio 2005 integrated development environment, application program was developed. Using MTi micro attitude heading reference system as the test device, synchronous data collection experiment was carried out on the SGT320E three-axis turntable platform together with a signal generator, a USB to serial port convert and notebook computer. Total 6156 data per channel were obtained from four serial ports by the development software at the sampling frequency of 10Hz. The total data from four channels were synchronous accurately according to the roll and pitch curves of the platform and the test device. The results indicated that the proposed synchronous serial port data acquisition method is feasible. 