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In order to overall monitor the vibration environment of missile storage, design a real-time monitoring system of shipboard missile vibration environment. It was made use of picking up the acceleration signal caused by the ship rolling, pitching, heaving motion. It used integrated and modularized designing idea, and applied PC104 bus to improve the system portability, adaptability. In addition, Two kinds of power supply was used to enhance the system reliability. This paper expounds in detail the design of hardware system and software system, and mainly discusses data acquisition and data display function realization. In the sampling frequency of 1000Hz, collecting data are accurate and reliable, accuracy of 99.75%, reaching the requirement of system design.