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Signal spectrum analysis was widely applied in the field of engineering technology, and it is very important to analyze the frequency spectrum of composite frequency signal. A new method of spectrum analysis using multi sampling rate was presented, which had high frequency resolution and low computing complexity. The spectrum of 20 Hz~20 kHz composite frequency signal was estimated by FFT algorithm using high sampling rate, and refined by down sampling the signal and FFT algorithm. A composite frequency signal cymometer based on TMS320F28234 was designed to sample the composite frequency signal which spectrum was analyzed by multi-sample-rate method, and the results were send to PC in real time which frequency resolution is 2Hz. 