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This paper introduces a novel design of achieving ARINC429 Bus protocol in one chip and synthesizing USB PCI in one interface board. According to the performance and technical indexes of the platform, the ARINC429 bus interface board has been designed. In terms of hardware, the modularization was adopted to design the system circuit; the functions of each part of the system were analyzed; the hardware interface was designed in detail. In the software design, this paper gave out the conclusion of design and achievement of NIOS II processor in terms of both hardware and software; the design and achievement of multi-channel bus protocol IP kernel from the aspects of overall design, transmission channel, receiving channel and interface logic were also introduced. Finally,the test of interface function was done. The test results indicated the designed ARINC429 interface board can work properly. This interface board can avoid the fixed data format of specialized chip, and It is expedient and reliable. 