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Infrared detection imaging and supplementary information collection, storage and analysis have been proved to be very important in Infrared imaging test system. According to the characteristics of infrared imaging detector system output information, a hardware and software design methods and implementation scheme of infrared detector based on PCI bus technology are introduced, which is also helpful in solving problems such as the real- time data acquisition, display and storage of various resolution infrared images. System tests are based on 320*256 and 640*512 resolution image accepting.Tests indicate that the read rate of PCI bus can reach 49.0MByte/s by using DMA. It can accurately implement high speed real-time sampling of various resolution infrared image and auxiliary information, which fully meet the user’s requirements of the testing system, and provides a solid foundation for infrared imaging detector system output sampling.