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Snake model in medical image segmentation has become increasingly widespread, but in the application of the model, how to select the appropriate initial contour is a problem. In this paper, we propose a method to extract the ROI’s initial contour based on the gray information of IVUS images .According to IVUS image’s grayscale features, firstly, adaptive thresholding and area filtering are used to reduce the complexity of the IVUS original images, then get splited contour points set and finally get the snake’s initial contour points set.Two kinds of IVUS images’ outer membrane extraction simulation experiments in Matlab7.0 environment prove that the initial snake contour obtained by this method is suitable for snake model’s iteration, as its initial contour is close to the true outline of the target, it saves more time and improve the efficiency of the algorithm compared with manual extraction method, it can be more easily applied to practical fields.