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The data acquisition and analysis system based on Java is designed and realized for achieving the data communication between the laser measuring instrument of wheel out-of-roundness and the serial port of computer. This system can realize the dynamic acquisition, display, analysis and processing and storage functions between the measuring data of wheel out-of-roundness and the serial port of computer. It has friendly man-machine interface and the characteristics of real-time,Sreliability and security. The testing results indicate that the data acquisition and analysis system based on Java provides technical support of the data acquisition and analysis of wheel out-of-roundness in the field.